A trained tree climber will climb the mother tree and collect young healthy shoots.
The plant material is sterilized to eliminate any bacteria, fungi, or viruses that may be present on the surface of the plant. The plant material is treated with a solution containing disinfectants and antibiotics.
The sterilized plant material is transferred to a growth medium containing nutrients and plant hormones. The culture is then incubated in a controlled environment with optimal temperature, light, and humidity.
After the culture has grown, it is transferred to a fresh growth medium to promote further growth.
The culture is treated with plant hormones to promote the growth of shoots. The shoots are then separated and transferred to a new growth medium for further growth.
The shoots are treated with plant hormones to promote the growth of roots. The plants are then transferred to a growth medium containing nutrients and plant hormones to promote root development.
The Tissue culture teak are transferred to a greenhouse or nursery for acclimatization. This process involves gradually exposing the plants to external conditions to prepare them for transplanting.